I am obtaining low yield when purifying IgG from patients with lupus. Any troubleshoot advise?
Hi Maria, protein G beads can be finicky. Sometimes you have to have 1M NaCl in the medium for the IgGs to stick.
Therefore, I recommend MEP resins from Pall to isolate IgGs
https://shop.pall.com/INTERSHOP/web/WFS/PALL-PALLUS-Site/en_US/-/USD/ViewProduct-Start?SKU=gri78m5c&_ga=2.183431205.2036721069.1498242337-1388438749.1481908196
Thanks! I shall try.
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