We are using the HEK293 FlpIn cells to generate stable isogenic cell lines of our GOI harvesting different mutations. When we conduct transient transfection experiments the expression levels (on protein level) are similar. Upon generating stable cell lines we are experiencing differences in expression levels (both on protein and RNA levels). While this of course could be due to the mutations I want to exclude that this could be due to a technical problem with the FlpIn system. I'm happy to get your experiences using these cells.
Thank you in advance for your comments!
Regards,
Gernot