How can I get rid of the bubbles in the SBS membrane?

I used methylcyclohexane/ ethylcyclohexane / n-butyl acetate mixed solvent to dissolve thermoplastic SBS & terpene resin. The mixture is then poured onto a glass substrate. After solvent...

24 January 2021 8,306 3 View

How do I get the permission for using the images of a published article?

How do I get the permission for using the images of a published article?

03 August 2020 9,083 3 View

Relationship between linear scanning voltammetry and electrochemical impedance spectroscopy?

I am currently running some electrochemistry experiments, including LSV and EIS. According to Ohm's law, the larger current responses the smaller impedance. What is the relationship between the...

27 November 2019 773 4 View

How to simulate the wick structure in a loop heat pipe?

Dear researchers: I am a newbee in the CFD simulation. Now I wish to simulate the wick structure by Fluent and the evaporation process in this region. The wick is saturated with water and being...

18 September 2018 9,281 12 View

What is a good algorithm in the operation research area?

Obviously an algorithm that may get better results than existing algorithm is probably a good one. But how to evaluate an algorithm that solve a new practical problem (or subproblem)? There are...

03 September 2018 7,799 2 View

How to compare effect size between two items?

Dear all experts, I'm currently doing a fMRI study and compare two groups in two conditions (switch and non-switch), and wondering if they have differences in one ROI (region of...

18 April 2018 2,789 3 View

What buffer I can use to permeabilize mitochondrial and fix the cell for immunostaining?

Dear All, My goal is to visualize a certain protein(known to be expressed in both cytosol and mitochondrial) in order to determine its sub-localization in mitochondrial. (Electron microscope...

08 April 2018 1,222 3 View

what difference are between cyclic voltammetry (CV) and cyclic polarization ?

When I studied electrochemistry and corrosion , I found cyclic voltammetry (CV) and cyclic polarization were similar curve shape, but analytic ideas were different. However many articles have...

01 February 2018 9,401 0 View

Can anyone give me some advice on mitochondrial preparation for microinjection?

I am trying to do somework about mitochondria injection, facing lots of difficulties about mitochondria preparation. I prepare the mitochondria using the method from the article of Frezza et al....

30 November 2017 3,514 1 View

Can anybody offer some methods for sorting good granulosa cells?

23 July 2017 8,973 2 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

How to estimate oocyte numbers in the mouse fetal ovary?

How to estimate oocyte numbers in the mouse fetal (1dpp) ovary?

23 November 2020 1,038 2 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

How fast is mitochondrial degradation by mitophagy in yeast?

Hi everyone, I hope everyone can help me with this. First, I am measuring mitochondrial membrane potential by flow cytometry using TMRE and MTG dyes. I think we are not experiencing problems with...

17 September 2020 8,748 4 View

Oocyte collection (GV and MII)?

Does anyone have a protocol for simultaneous collection of VG and MII oocytes in the mouse (from the same animal)? Thanks

06 September 2020 1,091 3 View