The effect of 0.05% Sodium azide on RNA extraction and quantification?

Dear RG users, I am dealing with a cervical sample collected with brush and placed in a specimen transport medium (contains 0.05% sodium azide) for HPV detection and genotyping. I have also a...

23 March 2020 1,107 3 View

How viruses evolved?

I have learned from different literature is that the origin of viruses is not well known though there are different theories on the subject. I am looking explanations from RG users on current...

04 March 2018 2,871 4 View

What are the different types of cancer vaccines currently being in use?

30 November 2017 6,056 3 View

Is quantitative data analysis is the most accurate type?

People think numbers are more accurate than words although the quality of data analysis matters. Which one do you thin is more convenient in applied research? What are the...

15 November 2017 6,182 5 View

Conserved regions of HPV genome?

07 July 2017 766 3 View

Rapid diagnostic (point-of-care) options for cervical cancer associated with HPV?

29 June 2017 2,917 30 View

Any article exploring weather rabies could be transmitted with contamination of open wound with saliva from rabid animal?

08 May 2017 3,263 3 View

Transformation of African Agriculture

If you were asked to make top three priorities to transform African agriculture, what would you propose?. List only top 3 critical interventions to be addressed.

01 January 1970 6,325 14 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View