Hello all,

I am new to cell culture work and would like to know if anyone has experience determining an appropriate drug dose for cultured neurons, astrocytes and microglia? (Derived from human iPSCs). Would this simply be a dose-response study and determine the smallest dose that elicits a measurable (and desirable) effect?

We are concurrently testing this new drug in a small primate; the dose is easier to calculate based on the recommended dose in patients (2.5 mg in human patients weighing on average 170 lbs; roughly 15 - 20 ug in a small bodied primate that weighs ~450 g).

If you have any experience with this and/or reference papers I can look at please let me know. Thank you very much.