I am monitoring the formation of an intermediate in a metalloprotein system where oxygen is an initial reactant. For sample prep I need to use a 20% glycerol solution; however, I know that oxygen solubility is different in glycerol than in regular water. Therefore I believe I need to determine what the concentration of oxygen will be in a 20% glycerol aqueous solution, rather than the normal concentration of oxygen in water at standard conditions (approx 220 uM). I'm not quite sure how to go about this though, I know Henry's law would be a start, but I think I'm confusing myself. Anyone able to start me on the math or have a good reference?
I also can stir under an oxygenated atmosphere to try and saturate my solution, but I think that is a different calculation
The Winkler method will work (https://www.youtube.com/watch?v=FKdzbgHaQQM). However, from experience, that determination can be tricky. Fortunately, others already did it, see the first attachement. Your value for the oxygen concentration is only approximate, you need to correct for temperature and ionic strength, and then multiply with a factor from the first attachment. For pure water, the values are shown in the table just above the references in the second attachement.
Good luck!
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