I would like to measure the concentration of NO in rat tissues. Is Griess reagent ok? It measures only NO2. Can I measure it also in samples from frozen and unfrozen tissues (not fresh)?
Yes, you can try.
But keep in mind that these compounds are very unstable and volatile and the less you freeze/unfreeze your samples the better will be your result.
I generally do the assay by the time I get the samples or by the next time I unfreeze them.
If possible use DAF reagent.. It measured NO in a fluoresence based manner..
I have tried Greiss reagent, DAF staining, and NOanalyzer before. Yes, you may try any one of it but just be aware to avoid nitrite/nitrate contamination when you prepare your working solution. I think Greiss reagent measures NO2 (nitrous oxide) is a mistake you must mean NO2- (nitrite). You can acutually convert NO3- into NO2- using cadmium method or E. coli nitrate reductase to covert NO2-/NO3- into all NO2- and measure it. Good luck.
Thank you for your answers. Of course, I mentioned NO2-, not NO2, - is missed somewhere. :-). Does this mean, if I would measure NO2- only with Promega kit, I would bring me no usable result? Is there no ratio between NO and NO2- and between NO3- and NO2-?
Hi,
you can also quantify NO by electron spin resonance (ESR).
In this link you will find further information.
http://www.sciencedirect.com/science/article/pii/S0300483X01004747
good luck!
Sandra
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