Who has analyzed essential oils in hemp and/or hops on HPLC?

I am trying to utilize my equipment as efficient as possible and want to try to run terpenenes (essential oils) on hemp and hops. Has anyone tried this before or does anyone know whom to contact...

17 August 2020 7,267 6 View

Where can I find academic books on sustainability and also graded/extended reading?

I am purchasing books from my designated budget. Academic books on either sustainability, UNESCO 2030 goals, and also graded and extended reading. Any suggestions would be very helpful and...

09 March 2020 4,723 5 View

Can a cross-over design control for regression toward the mean?

04 March 2020 3,302 3 View

Importance of light chain type on isotype control antibodies for flow cytometry?

I am putting together a panel for flow cytometry, for one particular antibody the only available isotype control has a different light chain to the test antibody. My test antibody is BV711-CD25...

10 June 2019 3,466 2 View

Can anyone explain how I choose a surfactant for a water in oil macroemulsion, and how I decide what volumes of each substance to use?

I am trying to create a water in oil macroemulsion with droplet sizes as large as possible (hoping to get between 0.1-1mm). I have no idea what surfactant to use or the volume of each phase to...

31 May 2019 7,376 3 View

I am planning to do a project management study that is related to health. What is the best topic to research on?

Any suggestions i will appreciate

06 April 2019 7,158 8 View

Optimising RT-qPCR for the detection of very low abundace IL-17A transcripts from human PBMC mRNA?

I am performing qPCR to quantify cytokine expression in human peripheral blood mononuclear cells. I have found it very difficult to get consistent results when assaying for IL-17A. Currently I am...

23 January 2019 8,404 1 View

Can DNase treatments to RNA preparations cause damage or loss to the mRNA?

When performing qPCR, I have noticed that cDNA made from RNA preparations treated with Turbo DNase (Invitrogen) prior to reverse transcription produce CT values that are shifted several cycles...

31 October 2018 9,688 5 View

Deaf friends in Dar es Salaam have asked: What research has been done on the signed language(s) used in Moshi and Arusha, Tanzania?

My Deaf friends in Dar es Salaam, Tanzania have told me the the sign language used in Moshi and Arusha is different than the signed languages used in other parts of Tanzania. They asked me to...

11 July 2018 3,549 1 View

Is a cell self-conscious?

10 September 2017 5,686 88 View

How to improve HPLC method to separate unknown sugar compound from the active fraction of marine echinoderm?

I had run HPLC for separating unknown sugar compound from a marine sample using various methods. The best chromatogram result i can obtain was the one in the attached picture. The method i had...

03 April 2019 7,585 1 View

Help please in taxonomic identification of marine macro-invertebrates?

Currently working on marine macro-invertebrates (especially molluscs, echinoderms and crustaceans) from gleaners' catch for my thesis and need help in taxonomic identification and confirmation....

01 February 2019 5,306 3 View

What is this little thing?

What is the species that is moving around the echinoderms larvae?

08 August 2018 2,470 4 View

What is this little thing?

What is the species that is moving around the echinoderms larvae?

07 August 2018 6,257 5 View

Where can I buy an universal anti-NOS?

Dear all, I would like to test an anti-nitric oxide synthase (NOS) antibody in echinoderm. I could read that the universal anti-NOS from thermofisher (PA1-039) was commonly used, also in...

03 April 2018 8,604 3 View

Crude extract of an echinoderm tested for antimicrobial activity, showed more activity in lower concentrations. Can any one help me to explain this?

Dried methanolic extract was used for assay, Broth microdilution assay was used, the higher concentration prepared by dissolving 10 mg of crude in 1 ml DMSO. 1:2 dilutions were prepared and made...

08 September 2017 9,874 4 View

Is there any simple procedure for Polysaccharide extract desalination ?

I would like to eliminate NaCl used in the extraction procedure that precipitate while precipitating polysaccharides with ethanol.

04 May 2016 6,891 0 View

Is there any ophiuroid specific universal primer for barcoding mitochondrial COI gene sequence?

03 April 2016 5,369 2 View

Echinoderms must be treated as movil or sessile organisms?

I need clarify if Cucumaria flamma or Eucidaris thouarsii that lives into holes must be considered Sessile organisms.

31 December 2015 255 1 View

What type of species (Echinoderms) is this ?

Collected from Palk Bay @ Low tide (7.30 am) on Feb 2013

13 June 2015 480 9 View