I had run HPLC for separating unknown sugar compound from a marine sample using various methods. The best chromatogram result i can obtain was the one in the attached picture.
The method i had used:
Column: Purospher® STAR RP-18 endcapped (5µm), brand new
Mobile phase: Solvent A : 0.1% TFA in H20
Solvent B : Acetonitrile
Temperature: Ambient
Detector : UV/PDA
Method: Linear gradient of solvent B from 0% to 50% in 30 minutes
Injection volume: 10 μ L
Based on the chromatogram, is there any reason for an increase in the baseline after the three peaks?
Would increasing the temperature or lowering percentage of solvent B provide better separation distance between the three peaks?
Hi @ Hasinah Rafiq
I have run the Thrmo UHPLC 3000. From my point of view you have choose a prefect solvent medium for this experiment. In short preform these test using the available solvents you have and control the Rt also. Perform all the experiments using the HPLC grade chemicals otherwise you will get noise.
Regards
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