How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How or where can I get the crystallographic information file (CIF) for PCN-222 MOF?

I need the simulated XRD pattern of PCN-222 to compare with experimental data. Thanks.

19 February 2021 4,932 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

How can I obtain the expansion orbital coeffiecients in Hartree Fock program?

23 December 2020 6,756 3 View

LINCS warning only with -pme gpu?

Dear all, I recently bought a RTX3080 and was unable to start the NPT and production steps due to lincs warning (my old gtx 1080ti card ran normally, using the same .mpd), I tried several...

18 December 2020 3,333 2 View

4D rotations and rotors.. can a general expression be derived?

I recently asked in math stackexchange a question regarding 3D rotations in geometric algebra https://math.stackexchange.com/questions/3922021/angle-and-plane-of-rotation-in-3d-geometric-algebra....

07 December 2020 5,630 26 View

What is the relationship between induction machine stator reactance variation and slip?

It is possible to notice, at some three phase induction machine datasheets, that exists a relationship between stator leakeage reactance and slip, that is, the stator leakage reactance increases...

23 November 2020 2,949 3 View

How to compute pressure drop on liquid ammonia line?

I have to analyze an existing ammonia line to estimate the pressure drop in it. The line is designed to transport liquid ammonia. I can compute the pressure drop with standard friction factor...

21 November 2020 5,494 3 View

Does anyone know of a qualitative research article that addresses the role of the CEO and/or Top Management Teams in ambidextrous strategies?

I am interested only in papers with a qualitative approach to explore the link between personality of top management team human resource management (including the CEO) and open innovation. Thanks...

19 November 2020 1,785 7 View

How to characterize the pore size of membrane filtration with bubble point method?

Hello everyone, I am having troubles with the bubble point measurement to find the pore size of my self-fabricated microfiltration membrane, and I couldn't find a proper answer anywhere else. It...

16 November 2020 7,574 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Can anyone recommend a scale to measure Bloom's taxonomy of learning styles?

I am looking for recommendations of scales (in English or Spanish) to measure learning styles according to Bloom's taxonomy of learning. Thank you in advance!

09 February 2021 1,419 8 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

What species (or genus even) of crab is this (bivalve using hermit type)?

Two weeks ago I found a peculiar crab in the intertidal zone in SW Florida. when found, the crab was not utilizing a shell. It was similar shaped to porcelain crabs found in our area, but with...

17 January 2021 1,620 4 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View