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How can I determination Bimatoprost in Eyelash Enhancing Cosmetic Serums?

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What is the effect of moisture of the atmosphere on titer of Karl-Fischer reagent (one-component)?

I have a Karl-Fischer reagent (for volumetric titration, which is a one-component reagent) with a titer of 5mg/ml. All references unanimously say the Karl-Fischer solution loses its capability...

02 July 2024 5,044 3 View

What is bibliometric article?

Greetings and Regards I have a question about bibliometric articles about public health or nursing. If any of the researchers have experience or knowledge in this matter, please tell me. Thank You

15 June 2024 2,795 1 View

Is there any guideline for method validation for cosmetic?

Hi everyone Is there any guideline for method validation for cosmetic? I wanna know about criteria for that.

31 May 2024 1,990 2 View

Which types of data can be used for lung cancer mutation detection?

I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...

13 May 2024 5,726 6 View

How can i prepare self healing hydrogel for MTT assay?

Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its...

11 May 2024 6,310 1 View

How can I extract and determination PAAs and PAHs in tatoo?

Hi Have a nice day I want to determination PAAs and PAHs in tatoo. Would you present and introduce methods to this?

02 April 2024 6,772 0 View

Is there a way to improve the fragility of polyethylene terephthalate after hot press?

"Hi, I gasify the polyethylene terephthalate granules and mold them using a hot press at a temperature of 260 degrees Celsius to prepare a bulk sample. However, after molding with the hot press,...

31 March 2024 8,668 0 View

How can I overcome the overfitting problem?

I have a dataset from lung cancer images with 163 samples (2D images). I use the fine-tuning of deep learning algorithms to classify samples, but the validation loss did not decrease. I augmented...

21 March 2024 8,344 6 View

Solvent for polyphthalamide?

Hello, I want to dissolve polyphthalamide in a solvent. I tested different solvents, formic acid, toluene, DMSO, alcohol-based solvents, etc. under high temperature and time, but it did not...

12 March 2024 3,030 1 View

Microplastics removal from wastewater?

Hi. I need to remove all microplastics of a wastewater sample without changing any physicochemical properties of the sample. Can filtration by Whatman filters (for example Whatman 589/3 with...

08 March 2024 8,722 3 View

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CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

Why do we use Horse Serum for C2C12 cell differentitation?

I have seen some papers that differentiate C2C12 myoblasts into myotubes by changing the growth medium that contains 10%FBS by a medium containing 2%Horse serum/calf serum + 1% NEAA. What's the...

04 July 2024 4,732 1 View

My cells need to be treated 4 times (0-72hrs) and then take conditioned media for LC-MS. Can I keep them in serum free for so long? Recommendations?

I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.

03 July 2024 4,044 3 View

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