Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below article.
Microscopic images of possible identified cases were taken with a magnification of 10*20 X, as follows.
Please guide us about the accuracy of detected eggs from other residues (flour) as well as the type of species (beetles) related to them?
Can this method be also used for the detection of larvae and pupa?
And
In general, what is the fastest way to detect and identify each stages of beetle life cycle (egg, larvae and pupa) in stored or processed products?
Have you ever checked X-ray?. Choosing that you may monitor a significant amount of the product and NOT just a single sample.
Kindly check https://www.researchgate.net/publication/235247751_Detection_of_eggs_of_stored-product_insects_in_flour_by_staining_technique
Also check https://www.researchgate.net/publication/7939350_Detection_of_Insect_Infestation_in_Stored_Foods
Periodical sampling and then identification on every stage.
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