Offshore steel structure of wind turbines 3D samples ?

i want to do a study on offshore wind turbines on sap model , initial study for graduation project of steel diploma (at ainshams university) , kindly if any specialized in this field can help me...

05 November 2020 3,571 4 View

Standarized vs. non standarized beta?

in the analysing the structural model in SEM using smart PLS , what is the standarized , and the non-standaraized beta in the outcome tables? cause in the path coefficient headline , there is...

26 October 2020 3,930 3 View

What to do if path coefficient is significant and f2 is no effect ? p and f2 contradicts?

in my phd thesis analysis through PLS for 574 sample size , is it fine to have significant path coefficient between A and B variables, but f2 less than .02 in the same time? specifically .189, how...

15 April 2020 2,728 3 View

Carbon weight percent in austenite?

Hello, I'm a first year student, we have a steel alloy that consists of 0.6 wt.% carbon, but when we measured the carbon content in austenite using XRD, we got 1.30 wt.% carbon. How can...

14 April 2019 9,870 4 View

How to deal with absent of standardization in methodology to carry-out new study?

Hi, I will be using image processing techniques to predict treatment outcomes to some kind of tumors. I'm about to design my study, So, I went through many papers and literature that has...

25 March 2019 2,348 2 View

How do I make my own DNA standard Curve for qPCR library quantification?

For library quantification before sequencing on Illumina, I use the KAPA library quantification kit with their included DNA standards. However, I need to quantify specifically mito or nuclear DNA...

03 March 2016 9,313 1 View

Can someone provide me with references about stormwater biofiltration structures at construction sites?

Do you have examples about publications discussing stormwater biofiltration during construction works? I think I have heard that if biofiltration or any other vegetated stormwater management...

19 February 2015 5,132 3 View

Has anyone used Genewiz for DNA synthesis?

02 June 2014 9,259 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View