Does anyone know what the best way to quantify PD-L1 expression is? Following treatment, I see a massive PD-L1 upregulation in mRNA level using qPCR and but only a partial upregulation using flow cytometry (done without permeabilizing- surface expression only). However, I see no difference in expression using a western blot (using PD-L1 (E1L3N®) XP® Rabbit mAb #13684). Has anyone come across similar issues? Any ideas on how PD-L1 is recruited to the surface?

Many thanks

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