Detecting fluorescent mouse strains without PCR genotyping?

More Navneet Vasistha's questions See All

The function [sech(z) - 1] is convex or starlike function?

23 April 2024 2,394 0 View

Why is the Bradford assay not working on solid BSA flakes sample?

Hello All, I have been using the Albumin standard ampules from thermo scientific of 1ml to make a standard curve for protein and I even got a nice standard curve. But when I am using the solid BSA...

10 March 2024 6,462 3 View

Present your research @ Scopus/UGC Approved International Conference on Interdisciplinary Studies in Education Research & Technology 2024?

Dear Colleagues, It is indeed a pleasure to invite you to International Conference on Interdisciplinary Studies in Education Research & Technology 2024 (ISERT – 2024)...

11 February 2024 3,728 0 View

I have designed a loop antenna in CST at NFC(13.56 Mhz) in CST. The designed antenna is working good at 3GHz. Why simulation stuck in MHz?

An error message is " Simulation takes longer time" and "Check your units and frequency", However, antenna dimensions at Mhz. Kindly give your valuable suggestions

31 January 2024 6,274 2 View

How best to perform statistical analysis of distributions?

Hi all, I have some calcium imaging data collected from neurons in the mouse cerebral cortex. I wish to compare the effect of two genotypes. Within each genotype, I have several neurons that...

25 January 2024 9,156 3 View

How many reads (25 or 50 million) are enough for differential gene expression?

I am doing an experiment on Cannabis sativa having a haploid genome size of 800 Mb. I have two groups (control and treatment) with 3 replicates each. How many reads should I go for? Does the sex...

24 January 2024 3,710 1 View

Present your research @ WoS/Scopus/UGC-Care International Conference on Interdisciplinary Studies in Education Research & Technology 2023?

Dear Colleagues, It is indeed a pleasure to invite you to International Conference on Interdisciplinary Studies in Education Research & Technology 2023 (ISERT – 2023)...

27 June 2023 3,556 3 View

Error in import output of SWAT file "String reference not set to an instance of a String. s"?

Dear All, Greetings I am getting an error in step of read SWAT output to import file to database “string reference not set to an instance of a string parameter name:s” Earlier, it gave an error of...

13 July 2022 1,520 0 View

Why normalization of matrices is needed in MCDM?

Significance of normalization Normalization is a process to transform data in different units into a common scale and comparable units. Why normalize in AHP: The first step in AHP is to assign...

25 May 2022 4,643 8 View

Computing larger values of exponential function in coding?

I am doing coding in python and fortran77. In both languages when I try to use exponential function (exp(x):where x is a real number). It returns 0 or infinity for the larger or smaller values of...

28 April 2022 307 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Paul Rutland

Amazon have them for instance

BORUiT LED Light Headlamp 5000 Lumens Rechargeable Blacklight Headlight for Night Fishing, Scorpions Hunting, Auto Oil and HVAC Leaks, Detect Fake Money/Jewelry/Pet Urine Stains (UV+White Light) : Amazon.co.uk: Sports & Outdoors

Amazon.co.uk: BORUIT

Navneet Vasistha

Thanks for that recommendation Paul. Have you or someone you know used it? How reliable is it?

Sorry I do not know how reliable it is but compared with most lab equipment it is pretty cheap Navneet but if cost is an issue then a mains powered bulb and fitment is probably quite cheap or even putting the mouse on a transilluminator but the torches are almost certainly better for the safety of your eyes