Navneet Vasistha

6 Questions 18 Answers 0 Followers

Questions related from Navneet Vasistha

Detecting fluorescent mouse strains without PCR genotyping?

Hi all, I am searching for a head mounted UV light based system which could allow me to identify fluorescent mouse strains without needing to run PCRs. Does anyone know if this is available? A...

15 November 2024 1,523 3 View

How best to perform statistical analysis of distributions?

Hi all, I have some calcium imaging data collected from neurons in the mouse cerebral cortex. I wish to compare the effect of two genotypes. Within each genotype, I have several neurons that...

26 January 2024 9,199 3 View

What is the best CRISPR/Cas9 method for simultaneous editing of multiple gene loci?

Hello genome editors! I wanted to know what techniques you use to edit multiple gene loci at the same time. I want to do this in ES cells and target up to 5-6 loci at the same time. Is there an...

26 March 2018 1,371 2 View

What statistical analysis could I do for a Sholl analysis experiment?

I'm comparing the arborisation of oligodendrocytes using the Fiji Sholl plugin and have 7 different conditions (cell lines) and variable number of cells counted for each line. Since no two cells...

17 July 2015 9,755 2 View

Does anyone have a transfection/lipofection protocol for neural rosettes?

I would like to deliver some reporter plasmids into neural rosettes without lifting them from the coating. I was wondering if anyone has a protocol for doing so either by viruses or by lipofection...

15 April 2014 1,987 3 View

Cre immuno doesn't work on free-floating sections?

I've been trying to get Cre-recombinase immunohistochemistry to work on mouse sections of a Cre-expressing line. I've tried two different Cre antibodies reported to work in other publications....

30 April 2013 720 23 View