Navneet Vasistha

5 Questions 17 Answers 0 Followers

Questions related from Navneet Vasistha

How best to perform statistical analysis of distributions?

Hi all, I have some calcium imaging data collected from neurons in the mouse cerebral cortex. I wish to compare the effect of two genotypes. Within each genotype, I have several neurons that...

26 January 2024 9,120 3 View

What is the best CRISPR/Cas9 method for simultaneous editing of multiple gene loci?

Hello genome editors! I wanted to know what techniques you use to edit multiple gene loci at the same time. I want to do this in ES cells and target up to 5-6 loci at the same time. Is there an...

26 March 2018 1,308 2 View

What statistical analysis could I do for a Sholl analysis experiment?

I'm comparing the arborisation of oligodendrocytes using the Fiji Sholl plugin and have 7 different conditions (cell lines) and variable number of cells counted for each line. Since no two cells...

17 July 2015 9,707 2 View

Does anyone have a transfection/lipofection protocol for neural rosettes?

I would like to deliver some reporter plasmids into neural rosettes without lifting them from the coating. I was wondering if anyone has a protocol for doing so either by viruses or by lipofection...

15 April 2014 1,936 3 View

Cre immuno doesn't work on free-floating sections?

I've been trying to get Cre-recombinase immunohistochemistry to work on mouse sections of a Cre-expressing line. I've tried two different Cre antibodies reported to work in other publications....

30 April 2013 648 23 View