I am doing an experiment on Cannabis sativa having a haploid genome size of 800 Mb. I have two groups (control and treatment) with 3 replicates each.
How many reads should I go for?
Does the sex of the plant matter as it is a monoecious plant?
I need to take the sample at 3 - 4 leaf stage and I won't know the sex of the plant at that time.
What should I do? Increase biological replicates? or its fine coz I'm studying stress response?