Hi Sakar,

Depends on what your research outcome is and the source of your DNA. For example methylation in the CG and CHG content is usually present on both strands (symmetrical) and thus the sense strand sequencing would be sufficient. Methylation in the CHH context, e.g. present in plant DNA, are strand dependent (asymmetrical) and you may want to consider the anti-sense strand as well for a complete methylation profile of your promoter. For example, the CHH context in a promoter of a transgene was most heavily methylated when introduced into plants https://www.frontiersin.org/articles/10.3389/fpls.2019.00312/full

thanks a lot