How to determine the DeltaCt for the BSP when you have two probes (fluorophores) for Methylated and Unmethylated?
I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...
03 February 2021 9,478 2 View
Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...
02 February 2021 10,047 3 View
I have a lack of knowledge on sample preparation for RNA extraction to test molecular diagnosis. I just want to know whether it is plausible to mix TCEP-HCl lysis/reducing buffer and NaI...
21 January 2021 9,309 1 View
Is the single nickel or cobalt atom attracted to magnet? Thank you.!
02 December 2020 5,584 3 View
I really want to study this, and I really wanna get some references or books about DG/HDG. I wish that you guys recommand me some good stuff.
26 November 2020 7,564 2 View
Hi. I am recently designing biomedical antennas. The problem is the permittivity difference between the skin and the air is too big, so I am having difficulties in delivering RF power to the...
22 October 2020 6,833 5 View
I'm interested in the research field of ion exchange resin. I want design about resin column with acryl for test. How do you usually design the diameter and length of small columns for test? I...
13 October 2020 3,443 2 View
I am using a SiO2 wafer with gold electrode on top of it, and I'm looking for a way to "re-use" the gold electrode if possible. Is there a way to not hurt the gold electrode (that is very thin)...
15 June 2020 8,300 4 View
Hi, I am trying to do codon optimizing for protein expression in E. coli. I used OPTIMIZER ( http://genomes.urv.es/OPTIMIZER ) before, but the website seems not able to connect now. I was...
18 April 2020 8,030 3 View
Hello. I have been trying to determine dissociation constant for a monoclonal antibody in solution using an ELISA-based protocol first described by Friguet et al.. The same protocol is also...
19 February 2020 1,212 10 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...
16 February 2021 8,389 3 View
Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...
11 February 2021 3,292 3 View
I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...
09 February 2021 3,981 1 View
I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...
09 February 2021 9,012 3 View
Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...
08 February 2021 3,366 3 View
Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...
04 February 2021 2,749 3 View
I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...
02 February 2021 9,065 5 View