How to determine the DeltaCt for the BSP when you have two probes (fluorophores) for Methylated and Unmethylated?
I have a group of combined variables to assess a technique for get the optimum prediction for breast volume before surgery? The independent variables comprises - number of markers, degree of...
21 June 2024 1,551 0 View
Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...
27 May 2024 9,144 1 View
Hi all, I am trying to use the 3devo filament maker to make my own filament. 1 I first dissolve the ABS pellets bought form 3Dxtech and then use ultrasonicator to mix the ABS and nano SiO2...
22 April 2024 4,433 2 View
I am currently conducting research on purifying Myoviridae phage particles using sucrose density gradient ultracentrifugation. However, I have encountered a challenge where no visible opalescent...
12 March 2024 9,387 3 View
Hi I am trying to simulate the crack propagation in 3 point bending by using Phase-field cohesive zone method, but I always have a convergence issue at 0.046s. Could you please help me? Here are...
02 March 2024 8,361 1 View
Hong Kong 2024:International Forum on Design Service & Social Innovation The purpose of this forum is to bring together knowledge sharers from within and outside the design field, to promote...
01 March 2024 7,847 1 View
JDSSI invites experts in the field of design and social innovation research to join the Editorial Board. If you are interested, please contact us or leave a message....
03 January 2024 1,734 0 View
My stock solution of Rotenone (Sigma) is 10mM in which 0.004g of Rot is diluted by 1mL of DMSO. I have done cck-8 assay on SH-SY5Y (2 x 10^4) with Rot treatments. Several articles show that 1uM of...
01 January 2024 3,430 3 View
Hi I am doing experiments with Rotenone-induced SH-SY5Y for PD research. I have seen some papers mentioning PD on their introduction but their methods with rotenone-induced SH-SY5Y do not...
28 December 2023 5,268 3 View
We used the DNA Clean & Concentrator-100 (D4030) from Zymo Research to clean and concentrate our 100 µg DNA. Unfortunately, our yield was only 8%. We initially believed that, due to our DNA...
18 December 2023 5,705 2 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,827 1 View
When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...
13 July 2024 7,131 0 View
Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...
08 July 2024 8,687 3 View
I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...
02 July 2024 900 1 View
If they can be acetylated and methylated what will be the effect?
02 July 2024 4,030 2 View
How to store SDS-PAGE for fluorescent imaging without fixatives? I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of...
29 June 2024 8,720 1 View