What effect do epidemics have on cerebral physiology?

How trauma and fear of infection can affect brain functions and structures?

10 October 2020 6,642 1 View

In your opinion, do I choose a certain disorder to study its relationship with culture, or better not to define the disorder?

Dear colleagues Who can help me find studies or references that study the relationship of culture to mental disorders. I would be very grateful for your help. :)

26 April 2020 3,003 36 View

Saffranin and fast green Staining ?

I need to stain the stem of lily flower (semi-herbaceous). is their any commercial staining that can be used directly. Also, Is their any updated procedure or commercial product (mixture) of...

18 January 2020 3,769 1 View

Minirhizotron images data set for root?

Hello, I'm working on olive study and I'm using minirhizotron images to estimate root density in soil profile. However, the image analysis software provide an extra data such as, root surface...

19 November 2017 5,621 3 View

Aquapoinic culture system?

Hello, When we use the aquapponic culture system, does the water that come out from the fish tanks has all nutrients (Macro and micro) that the plant need or should we add some? Thanks

11 November 2017 3,224 4 View

What are the Academic and Practical motivations for linearization of a non-linear mathematical formulation?

Hi, I am trying to write the motivation part for linearization of a non-linear mathematical formulation. I claim the mathematical model will be more simple and easier to solve. But, of course, I...

09 October 2017 2,284 3 View

Good day for all, please, any suggestions, in gel electrophoresis there is no band appear only the dimers?

I work on RNA virus and measure the conce. of RNA and cDNA by nanodrop there is good concentrations, but in gel electrophoresis there is no bands appear only the dimers?

16 April 2017 3,316 7 View

Chronic Insomnia from SSRIs like Prozac and Cipram?

29 January 2016 2,640 3 View

In PCR, Are the faint smear effect on results of PCR product sequencing?

with electrophoresis appeared the faint smear with specific band, are this smear interfering with the results of sequencing

01 January 1970 7,210 7 View

What is the relationship of electromagnetic poisoning with the destruction of hemoglobin and the epidemic?

To what degree can electromagnetic poisoning cause harm

01 January 1970 2,781 4 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 2,767 6 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Bacteriophages very effective against infections. Some bacteriophages can be oncolytic and effective against cancers. How B_P recognize cancer cells?

Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...

03 January 2021 4,769 1 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

15 November 2020 7,488 4 View

What are measurement criteria of bacteriophages in TEM pictures?

I've got the TEM pictures of my purified bacteriophage whose host is S. aureus. there are various shapes with tail/tailless (maybe a little tail). in order to place it in a family, I need the...

15 October 2020 5,261 5 View

How to submit annotated genomes to NCBI?

Hello, We would like to submit annotated bacteriophage genomes to NCBI but we have a bit of a hard time to fully understand the process. We already created the bioproject and biosamples and...

07 September 2020 715 3 View

Recommendations on model enveloped viruses that are easy and safe to propagate?

Hi everyone, We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact. In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to...

08 August 2020 6,731 4 View