I am trying to extract bacterial DNA from paddy soils using thermal shock and got this result. Was wondering if this is a degraded DNA or RNA?
Hi, by the look of it, whatever it is, it's definitely degrated. If it were RNA you should see the 2 characteristical bands for ribosomal RNA (18S y 28S), and way upper than this.
Here's a photo of what RNA should look like.
Hi, The gel showed degraded material.
Please refer link for guide:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140122/
http://www.biotechniques.com/multimedia/archive/00232/12888_232066a.pdf
https://www.hindawi.com/journals/mbi/2014/518960/
http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/Genomic%20DNA/UM_gDNASoil.pdf
http://www.bio.vu.nl/~microb/Protocols/Manuals/PowerSoil_DNA.pdf
http://www.earthmicrobiome.org/protocols-and-standards/dna-extraction-protocol/
Thanks
Thanks Yoke, but I'm looking for protocols that do not use of beads and kit for DNA extraction
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