Hi
My team and I are trying to design degenerated primers in order to sequence a viral genome. We do not know how many degeneration we can include in each primer. Is it possible to add up to 6/10 degenerations in primers of 20-24 nt?
Thank you.
Last time I tried as many degenerations as you describe, my PCR did not work.
Thank you for your help
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...
04 July 2024 9,001 0 View
Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...
30 June 2024 9,594 2 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...
28 May 2024 1,105 3 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...
19 May 2024 5,171 3 View
What is recommended as a strong and current reference of qualitative phenomenological analysis ?
16 May 2024 2,142 1 View
I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)
09 May 2024 6,543 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,666 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,113 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,483 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,672 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,404 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 840 2 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,952 1 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View