Is ''gene construct is a segment of DNA (T-DNA ) to be introduced in a target organism'' or does it ''includes the transformation vector DNA also i.e., 'segment of DNA to be transfer + the remaining vector sequence'.''?
What in case of a Double T-DNA harboring transformation vector (a vector containing two separate segments of DNA to transfer), will it be called to have two gene construct or two gene cassettes?
The gene construct is a complete set of cloning or expression components of any vector (expression /cloning) with your interest of gene in right orientation (in the case of expression).
Interest of gene cloned in choice of vector
A gene construct is a gene of interest associated with the regulatory sequences which is inserted in the right orientation in an expression vector. You can insert more than one gene construct in a vector when needed.
A gene construct contains all the information needed for transfection into a bovine cell and expression of the desired gene in a cow. This includes an antibiotic resistance marker, a tissue-specific promoter, the transgene/gene of interest and a stop sequence.
http://www.cgm.northwestern.edu/cores/ttml/transgenic-projects/transgenic-construct-design.html
For your question: " a Double T-DNA harboring transformation vector (a vector containing two separate segments of DNA to transfer)", I would say, "my construct has two T-DNA cassettes on it".
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