Govind Kumar Rai

4 Questions 10 Answers 0 Followers

Questions related from Govind Kumar Rai

Definition of a Gene construct?

Is ''gene construct is a segment of DNA (T-DNA ) to be introduced in a target organism'' or does it ''includes the transformation vector DNA also i.e., 'segment of DNA to be transfer + the...

15 June 2017 4,406 6 View

Which plant should be used as control for normalization to calculate Fold Change Expression of a transgene in stressed transgenic plants?

While calculation of the fold change expression of a transgene/gene, the expression level of target gene is normalize by subtracting delta Ct of treated sample with delta Ct of control sample....

29 March 2014 3,230 2 View

What will happen if water is added to wash DNA pellet instead of 70% Ethanol? Do the DNA solubilization continues during immediate centrifugation?

After precipitating DNA, if washing processed with water instead of 70% ethanol, then one will be able to recovered DNA immediately or it will get away with water. Do the DNA will get completely...

09 October 2013 2,045 7 View

How do you estimate the water use efficiency of plants?

What is water use efficiency? How Water use efficiency of plants can be measured? How a particular soil moisture condition can be maintained? How yield efficiency of under drought stress can be...

19 September 2013 8,235 44 View