The reverse primer is there but all papers publish primers as 5' to 3'. In your PCR product you should look for the reverse complementary sequence.

In your case (5-TGCCCCAGGGAACAAAGT-3) is located in the right side of the fragment as ACTTTGTTCCCTGGGGCA.

thanks dear, but what is the sequence sent to company to receive as reverse? 

Sequence send to company should always be both (forward AND reverse)  5' to 3' (like published in the paper)

good luck!

Exactly what Annemarie said, you always order oligos as 5' to 3'. And when you publish your data you also use the same approach!!!

Thanks dears

- Annemarie Bungartz.

 - Andre Fernandes.

for nice and good helps

but I hope continuation of your helps and nice collaboration to gate a best.

WARM REGARDS.