Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

Has anyone optimized ViromerRED in THP-1, H9, and MCF7 cell lines?

I am currently attempting to optimize ViromerRED transfection in THP-1, H9, and MCF7 cells. Our initial transfections with the first batch was promising, but the new viromer we received doesn't...

15 October 2019 5,053 4 View

What's the best way to confirm raised antibody levels in mouse serum after injecting the same antibody?

I just want to know that after in vivo antibody injection, the levels of antibody are higher several hours post-injection compared to several days post-injection. I'm not sure if antibody...

28 July 2019 8,334 3 View

Is there any treatment available for people diagnosed with alexithymia?

Anyone have information on treatment that has been successful for people diagnosed ( or who feel they have) alexithymia? Also info about interoception and alexithymia. Many thanks Jane

19 November 2018 8,822 2 View

Where can I get a Cas9 virus of known viral titer to use for a standard curve for qPCR?

I've made a Cas9 virus using the #60957 plasmid from Addgene, and I need to titer it using qPCR. There's no GFP reporter, and I'd prefer not to target the AAV ITRs because there are two of them...

08 May 2018 6,005 3 View

Formula of the punching force in the case of drawing a sheet with a hemispherical punch

01 June 2017 8,473 4 View

Does anyone have any Santa Cruz antibody for EGFR (1005), product number sc-03?

They've discontinued this polyclonal antibody it and we'd like to repeat some key experiments! We'd be willing to compensate you for it and pay for the shipment. Any help would be greatly appreciated!

06 May 2017 4,389 3 View

Does the hot mounting process have an effect on the microstructure of the specimen ?

26 April 2017 2,098 8 View

If a peer reviewed journal refuses to investigate a reported fraud with regard to a published paper, what can one do to remedy?

02 February 2017 994 17 View

Labelling monoamines with dansyl chloride?

Hi, I'm trying to observe where dopamine localises at the cell surface of my cells of interest but fluorescent dopamine is very expensive to buy. I've read online that I should be able to label...

12 October 2016 5,404 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View