Dear experts , i would like to know about selecting Tm for primers.

03 March 2016 1 148 Report

The company details show at least 2-4 0CTm more than the calculated Tm as per PrimerBlast. Which Tm i should take in consideration. Thanking All

Dr. Jamsheed Javid

Thank you very much Dr. Paniz

I used to consider the Tm of primers provided by the company, and it works well.

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