How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

02 December 2020 9,424 5 View

Why CD4 Treg lose FoxP3 during expansion in vitro?

Hello everyone! I have the following question: I tried to expand CD4 Tregs in vitro to get enough material for WB or any other application. This is my first trial with them. So, I`ve got FoxP3+...

13 September 2020 6,458 6 View

How should I care of mice after sublethal irradiation?

Hello dear colleagues I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to...

22 July 2019 1,844 3 View

Do exist any approved to human (or, at least, near to be) anti-inflammatory drugs, alternatives to NSAIDs?

09 August 2017 2,919 7 View

Classifier robust against non-informative features?

I have two feature sets I evaluated using SVM on a two-class problem. The first set (the "good" one) provides acceptable results, while the second one is far behind. When I merge the two sets...

12 March 2014 161 3 View

How do you specify CPSET and NSET for history output?

The simulation, I am trying to bring about, is essentially related to a contact analysis between deformable and analytical rigid bodies, the interaction between them is defined by means of surface...

13 February 2014 1,142 1 View

Dear colleagues! Do you think that all the team roles according to the Belbin model* will remain unchanged in the future?

Perhaps, some will cease to be relevant and, conversely, new ones will arise? And if the changes are still there, then what and why? (*Belbin model =...

01 January 1970 9,973 4 View

Transition from academic to business career: mission impossible?

Dear Colleagues! As far as, from your point of view, it is possible (and justified) to implement the concept of Life Long Learning in a professional academic career by completely withdrawing from...

01 January 1970 4,724 15 View

The need to disseminate information about the results of their scientific activities for the researcher in modern conditions.

Dear Colleagues! Do you think a modern researcher should independently make efforts to spread awareness in the world around him about the results of his scientific activity? If so, how much will...

01 January 1970 9,054 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View