Dear all, i want to get the protocol for isolation of Lumpy Skin Disease Virus from ticks by Pathogen Free Egg?

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Mohammed J. Al-Hilfi

I did not understand your question but on the subject of the pathogen if you want isolation from tick eggs

Ramy Essa El-Ansary

Dear Dr. Mohamed thank you for reply.

I want to get the protocol for isolation of Lumpy Skin Disease Virus by tissue culture and pathogen free eggs

Md Zulfekar Ali

yes you can isolate LSDV by SPF embryonated chicken. You may follow chicken pox virus isolation protocol for LSDV like CAM route.