"Double" bands after PCR

Hello everybody! I used new primers for the first time. As a result I had a "double band". Look at the line # 2,4,6,7,9. I think that 1st line band is the same, but it's too intense so we can't...

02 March 2012 6,311 61 View

Calculation of the amount of water needed to dissolve primer

I have to dissolve lyophilized primers and get a solution with concentration 100 mkM. What quantity of water should I use and how to estimate it? Primer quantity (OE) is 7.0; Mol.weight:...

01 February 2012 8,233 9 View

When I chek the quality of extracted DNA by means of agarose gel electrophoresis I have long band that is lower the DNA band. I don't understand what...

When I chek the quality of extracted DNA by means of agarose gel electrophoresis I have long band that is lower the DNA band. I don't understand what is it and how to avoid it. Unknown bands...

01 February 2012 8,364 26 View

How to dilute primers for PCR? In water or in TE buffer?

What is the most appropriate proportion of PCR composition, particularly for amplification of fish DNA?

01 February 2012 7,746 23 View

Problems related with origin of life! What was the first: DNA or RNA?

Waiting for your comments, tips, articles!

10 November 2011 2,444 20 View

What was the first?! DNA or RNA?

There are many point of view related on the origin of life. But DNA from RNA or vice versa?

10 November 2011 9,539 31 View

"Life is duplication with mutation". What about DNA mutation?! What cell elements are responsible for mutation?

Polymerase mistakes? Why not. But do we have the right to say about mistake? It is precisely because of an enzyme error mutations is occurring. What else can cause any DNA mistakes (mutation)?

09 October 2011 1,501 0 View

Hi to all! I would like to study PCR and sequencing methods abroad. Do you know any research centers or labs where I can master these methods?

A.g. studying during a month. Lectures and practice. Something like an express courses.

08 September 2011 1,600 17 View

Hi to all! I would like to study PCR and sequencing methods abroad. Do you know any research centers or labs where I can master these methods?

A.g. studying during a month. Lectures and practice. Something like an express courses. My address is [email protected]

08 September 2011 6,838 1 View

Hi all!I'm looking for the most appropriate device for DNA concentration measurement. I've heard about micropipets that work as spectrophotometers.

I'm waiting for your comments!

08 September 2011 5,155 19 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Statistical methods for comparing gene expression curves?

Hi all, I have generated two gene expression curves by measuring bioluminescence every 10 mins for 12 hrs. These curves tend to overlap at at some time points, while a difference can be seen at...

02 February 2021 2,613 7 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

New knockout bacterial strain: Can't electroporate it?

I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...

01 January 2021 1,580 4 View

Can you advise reference publications/standards on characterizing plant genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...

31 December 2020 5,871 4 View

What is the role of fresh cow dung in sprouting in stem cutting propagation?

In my personal experience I have find the higher rate of sprouting when fresh cow dung is applied on the top side of cutting what might be its reason.

24 December 2020 4,068 6 View

What is the level of consciousness , communication and feeling in plants ?

Do you think the plant can communicate with each other, what is the level of feeling in plants?

22 December 2020 2,754 17 View

How to cut dsDNA chain with Uracil-DNA Glycosylase at the point of dU in room temperature?

18 December 2020 6,908 3 View

Why pink coloured or light red onion have more storage life than white and dark red one and smaller size one to has more than bigger one?

It is generally seen light coloured varieties has more storage life.what's relation with colour and size with storage life.

13 December 2020 634 18 View

Can LAMP distinguish the species from different genus/family?

I am usiing LAMP kit, specifically designed for liriomyza huidobrensis. I have a random samples of differet insects and LAMP seems to amplify insects from other Genus as well. Although, other...

07 December 2020 5,630 3 View