I have done SEM for my self-assembled peptide and I got these images! Any suggestion/ idea about the shape!size!structure?
However, images are not opening at their normal sizes but i would like to confirm whether all three images are from the same self-assembled peptide or they are different samples?
This product may occur due to variations in process variables or environmental factors. Therefore it is not assured that none of these factors.
Similar cases were observed in the production of nanoparticles.
Thanks all for your reply! It is the same peptide with different magnification power! using gluteraldehyde or without!
Thank you for your reply! what do you recommend me to do so I can get better images?
To get better image you just simply keep trying. This is simply a matter of experience. the more you do it will be easier to get the desired results.
After you get the best image you can get from SEM, you can use software such as ImageJ to process your image.
http://imagej.net/docs/guide/146-29.html#toc-Section-29
Tri Hadi Jatmiko ! many thnx for responding
The first image is looking like disk like aggregates.
DOI: 10.1021/acs.langmuir.5b03987
Go through this article.
Thank you Baddi Sravan for your answer. I will
I have engineered peptide polymer based NPs and want to calculate the number of peptide sequence per NP. I know the concentration of N-terminal amine using fluorescamine. I know the average size...
10 November 2018 7,759 0 View
I am designing a delivery system using PEG-peptide conjugates. The peptide has the ability to self-assemble due to presence of a fatty acid or GF3 (tetra-mer). I dissolve the conjugates in nano...
07 August 2016 7,296 4 View
07 August 2015 5,535 14 View
11 December 2014 8,157 8 View
I am trying to purify a recombinant His tag fused protein from E.coli. I am facing several problems after I sonicate, centrifugate and purify it using His trap Ni column: 1- I got too much...
08 September 2014 8,284 9 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View