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Questions related from Safaa Kader
I have engineered peptide polymer based NPs and want to calculate the number of peptide sequence per NP. I know the concentration of N-terminal amine using fluorescamine. I know the average size...
11 November 2018 7,783 0 View
I am designing a delivery system using PEG-peptide conjugates. The peptide has the ability to self-assemble due to presence of a fatty acid or GF3 (tetra-mer). I dissolve the conjugates in nano...
08 August 2016 7,319 4 View
I have done SEM for my self-assembled peptide and I got these images! Any suggestion/ idea about the shape!size!structure?
10 October 2015 9,328 9 View
Hi all, Kindly, I am looking for a SEM/TEM`s protocol used for measuring size of self-assembled peptides (nano-structure)
08 August 2015 5,567 14 View
Would you mind suggest me some techniques to maintain my fused proteins` stability and function? My proteins are fused fluorophore proteins loosing their fluoresence substantialy at +4c. Also, is...
12 December 2014 8,186 8 View
I am trying to purify a recombinant His tag fused protein from E.coli. I am facing several problems after I sonicate, centrifugate and purify it using His trap Ni column: 1- I got too much...
09 September 2014 8,306 9 View