Dead Cas9 results from one mutation in RuvC domain (D10A) and other mutation in HNH domain. Is the second mutation H840A or H841A?

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How to do usability testing of sound?

Hi, What all parameters should one test for when it comes to sound? Is there any heuristics available? Thanks

19 July 2023 3,009 1 View

OTT and Virtual reality: Any literature around this?

Any literature available around the intersection of OTT and virtual reality?

04 April 2023 767 0 View

Generalising research results from one domain to another?

Hi, I am testing different levels of product/system training to participants (independent variable), with task performance on the product (dependent variable). Can I generalise the results of...

16 September 2018 2,387 4 View

Any open source/free software for summarizing cursor movements or heat maps generation for the cursor movements?

Hi, I have a recorded video in .mp4 format which has cursor movements in it. This was done using a video screenshot software. I need to have a static summary of the cursor movements made on the...

12 September 2018 7,451 2 View

Heuristic evaluation for low fidelity prototypes?

Design problem- If we have 3 UI concepts: A, B and C and we would like to quantify which one is better out of the three, can heuristic evaluation be used? Constraints: only low fidelity prototypes...

15 March 2018 1,653 7 View

Motorcycle HMI usability testing?

Hi, I am planning to conduct usability testing for a motorcycle HMI. The HMI has a joystick which can control the menu of the HMI (only when the biker is not riding the bike). I am thinking of...

31 December 2017 6,858 6 View

Revised HED-UT scale

Hi, For measuring UX, I have used Revised HED-UT scale by Heijden et al. It has 9 items for utility construct : useful, practical, necessary, functional, helpful, efficient, effective,beneficial...

27 June 2017 1,751 3 View

Will some one please clarify the two terms "genome-scale engineering" and "multiplexed genome engineering"?

The two terms "genome-scale engineering" and "multiplexed genome engineering" are not clearly differentiated in some scientific papers. It is important to know whether these two terms are...

13 June 2016 5,935 3 View

Method for assessing workload on body

Hi, I am trying to use Rapid Upper Limb Assessment (RULA) method for assessing the physical workload due to gestures and body movements based on a stimulus. I am referring to the following link...

01 January 1970 9,289 6 View

Is it feasible to employ an artificial sun in the experimental investigation of concentrated solar power systems, specifically linear Fresnel systems?

Is it feasible to employ an artificial sun in the experimental investigation of concentrated solar energy systems, specifically linear Fresnel systems? If the answer is affirmative, what type of...

22 July 2024 9,710 6 View

Every star like the sun is at a stationary position. Am I right?

The earth has a orbit, but not for the sun. Similarly, all planets have orbits, but not for stars like the sun.

18 July 2024 1,768 4 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

What do you think about the shining?

"Surya Pradana asked What do you think about the shining?" is a thought-provoking question that encourages us to reflect on the concept of shining or radiance in various aspects of life. The idea...

11 July 2024 9,513 0 View

What is the connection between the "proper energy" of a particle, the "limit energy" of a particle, and the "intrinsic energy" of a particle?

The “intrinsic energy” of a particle is well known, it is mcc so what about of the “self energy” of a particle if one want to call it so and the “limit energy” of a particle if one want to call it...

24 June 2024 3,366 3 View

As the main cause of climate change, why don't scientists show the main forces affecting the earth's rotation?

Scientists show that the main cause of climate change is the effect of greenhouse gas emissions.However, one of the main forces affecting the movement of the earth is the effect of hot uneven flow...

19 June 2024 1,174 2 View

Hi, How can I design primers to obtain Deletion mutants by Site Directed Mutagenesis?

I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...

05 June 2024 9,430 1 View

How can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are there any ways to increase the rate of HDR?

Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...

30 May 2024 9,993 0 View

Which amino acids to mutate to avoid proteolysis?

We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.

25 May 2024 7,649 0 View