The two terms "genome-scale engineering" and "multiplexed genome engineering" are not clearly differentiated in some scientific papers. It is important to know whether these two terms are synonymous or different.
Genome-scale engineering: Sequence changes to at least two distinct regions of a genome.
Multiplexed genome engineering: Large-scale (many) changes to single or multiple genomes by replacing large contiguous sequences or modifying numerous smaller sites serially or in parallel. This would require a high targeting efficiency.
These terms refer to novel approaches in strain engineering.
GSE: means that you consider the entire genome to engineer a cell in a systems-wide manner towards a superior cell factory
MGE: means that you manipulate genomic DNA massively in parallel, i.e. that you introduce your (many) changes of interest simultaneously. this saves a lot of time, when introducing a set of genetic modifications. as correctly stated by John, targeting efficiency is a crucial parameter. side-effects may combinatorially explode in number in case your modification is not completely precise. simply spoken, at the moment enhanced speed comes with reduced precision.
best,
christoph
Thank you John. Thank you Christoph. Your descriptions have been very helpful to me and hopefully will be helpful to others as well.
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