30 January 2018 0 7K Report

We are looking for colleagues who work with Cytation live imaging readers. Our Cytation 3 has failed due to overexposure by the LED bulbs? We would like to know if other users of the device were faced with this same problem. Did pasting of a membrane filter, as recommended by the manufacturer, restore the function completely and permanently?

Does the installation of a membrane filter solve the problem of, so that the device can be rebooted?

More Petra Stahlschmidt-Allner's questions See All
What are the experiences of nurses providing palliative care at home?

Hello What are the experiences and perspectives of nurses providing nursing care at home and in the community for patients in palliative care?

12 June 2024 2,236 5 View

Policy when missing my name on a presentation?

A joint presentation I am involved in is published on researchgate without my name. What is the policy here?

29 October 2023 256 1 View

Does anyone know the genotyping protocol for roy a9 and slc45a2 b4 zebrafish mutants?

Hello, I would like to ask you for help. I am trying to find genotyping protocol for roy a9 and slc45a2 b4 zebrafish mutants. Does anyone know them? Thanks.

26 October 2023 4,046 0 View

Is it possible to merge my two accounts?

Dear administrator, before I started working for the Uniklinikum in Erlangen, I was employed in Hamburg, Uniklinikum. After the long maternal leave I forgot I had an account already. I would like...

14 August 2023 1,483 2 View

Can I do my Master thesis specified to a retrospective of a company?

Hello all, appreciate your help as it is much needed. I am trying to understand whether my thesis should be a research paper or a case study. I want to study the effects of disruptive events of...

09 July 2023 1,933 6 View

How can I remove non-speciffic staining in free floating mouse brain sections labelled by immunofluorescence?

Dear all, I would appreciate a lot if anyone could give me an advice how to resolve problem with non-speciffic labelling of immunofluorescently-lablelled mouse brain free floating sections, which...

03 December 2022 9,604 2 View

What is the most accurate way to measure pH of deep anoxic lake sediments from a fresh core in the field?

Dear colleagues, from a practical point of view, how do I accurately measure sediment pH of more or less compacted, anoxic lake sediments in the field? Imagine there is a freshly recovered and...

28 July 2022 2,012 8 View

What is the best protocol to freeze MM28 cell line?

If any of you have worked with the MM28 cell line (uveal melanoma metastasis) how did you freeze it to maintain the best cell viability?

08 May 2022 4,400 1 View

Tools for risk-based groundwater management ?

Comprehensive ground water management requires suitable tools that consider the groundwater balance as well as the variety of potential risks that might impact groundwater recharge and chemistry....

30 December 2021 6,849 3 View

(Yeast) Do you have experience in using bead beater instead of vortexer for DNA purification before two-dimensional agarose gel electrophoresis?

I am currently working doing a lot of N/N 2D gel electrophoresis for investigating replication structures in yeast. We might invest in a bead beater, which can handle a specific volume, but I am...

21 September 2021 2,596 4 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close