I need to know how to identify cyanocobalamin by HPLC? I tried the USP method but I don't have good separation.
cyanocobalamin i.e. Vitamin B12. There are so many methods in literature. Please find attachment for following papers.
Another paper.
With PDA detector you can compare UV spectrum with reference - it is sufficient indentification procedure. Example spectra can be find here:
http://omlc.ogi.edu/spectra/PhotochemCAD/html/036.html
just determination of cyanocobalamin is very simple by HPLC, as sone paper are proposed by Robin Joshi..are very good.
but actually its very difficult to differentiate between cyanocobalamin and mecobaalmin simultaneously.??
Thanks Asad and Robin
You can easily use HPLC with UV detector at 361 nm and solvent system of methanol/water
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