At which positions these enzymes cut the plasmid pGFPuv.?
BamHI, NdeI
how to Calculate how many fragments of which sizes you can expect after each of the reactions.?
what is the pipetting schemes for the restriction enzyme reactions?
If you spend 5 minutes in google you will find multiple sites that give the sequence (and map) of pGFPuv. Copy the sequence and plug it into Webcutter or NEBcutter or any other free DNA manipulation webapp/program (many out there) and you will find that cutting with BamHI will cut at 264 and 808 and NdeI will cut at 517 and 1281
I do not know what are you trying to do but will mention both enzymes cut inside the gfp gene (289-1005)
and you should write down the pipetting scheme by looking into the manufacturers instructions of the enzymes you want to use
You need a software to know where your enzymes of interest will cut plasmid pGFPuv.. Like those suggested by Prof Yoram Gerchman and for the pipoetting follow manufacturers prescriptions
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