Liver toxicity by paracetamol and LPS using liver slices?

Negin Rahimi @Negin_Rahimi

20 December 2021 1 2K Report

I am trying to determine whether these statements are true or not

LPS toxicity is mediated by the toll-like receptor

NO is mainly produced by Kupffer cells

Liver necrosis is the main result of paracetamol toxicity

cholestasis is the main result of LPS toxicity

Glucuronyltransferases are involved in the GSH conjugation of NAPQI

sulfurotransferases are involved in the GSH conjugation of NAPQI

The liver is the main target of paracetamol because it has a high concentration of COX enzymes which are the target of paracetamol pharmacological activity

the liver is the main target of paracetamol because sulfurotransferase expression is generally low

GAPDH is used to normalize the concentration of gene of interest

The number of cycles required to have a sufficiently detectable amount of fluorescence in the PCR is dependent on the original mRNA concentration

Md Turab Hossain

Negin Rahimi Liver damage results not from paracetamol itself, but from one of its metabolites, N-acetyl-p-benzoquinone imine (NAPQI). NAPQI decreases the liver's glutathione and directly damages cells in the liver. Diagnosis is based on the blood level of paracetamol at specific times after the medication was taken.

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