How do wood moisture meters function when applying on live trees?

Eleven months ago I girdled 17 trees and measured the moisture content, at the place where they had been girdled, with a wood moisture meter. Typically, the moisture meter showed a reading of...

26 December 2020 7,761 2 View

Statistical test: 1 nominal independent variable and 1 dichotomous dependent variable?

I have a dataset with one nominal independent variable with 10 different levels and one dichotomous dependent variable. What would be the appropriate statistical test to compare the different...

09 December 2020 8,834 29 View

Where can I find a more pure source of trichloroacetic acid (TCA)?

I am analyzing nucleotides from TCA-precipitated cell extracts by HPLC coupled to UV detection (270 nm) and there is an interfering peak coming from the TCA itself. I have tried some sources of...

07 June 2020 387 4 View

WIll conservation of impulse from weight drops on floors with mats lead to a higher low frequency vibration level in the floor slab?

Weight drops generates vibrations in the floor they hit. Impulse mdv must be conserved, even if the floor or mass is cushioned with mats. Cushioning leads to less high frequency content in the...

17 February 2020 9,807 3 View

How can ion pairing agents cause peaks to split?

I have previously made a lot of nucleotide analyses with tetrabutylammonium as ion pairing agent but decided out of curiosity to try primary amines instead and then made a strange observation. One...

03 October 2019 6,864 13 View

We are running a citizen science project on tree- and forest lines. Are there any others out there doing the same?

We have made a mobile app for registration of tree- and forest lines in Norway. We would like to compare our results with similar projects. You can see the results here:...

03 September 2019 1,972 3 View

How does the solubility of the buffer in the stationary phase affect resolution in ion-pair RP chromatography?

I analyze nucleotides with tetrabutylammonium ions as ion-pairing agent on a C18 column and I have made an observation that the resolution of the peaks often decreases when I use buffers that I...

27 August 2019 2,030 1 View

How do I know that the fittings in HPLC are optimal?

I usually do not get as high plate numbers as claimed by the manufacturers and been thinking about how I can know if the fittings (nuts + ferrules) are perfectly seated or not. I have used ACE and...

16 July 2019 5,867 15 View

Bottle but not textile PET recycling - why not?

PET bottles are conveniently recycled into fibres by remelting and spinning. How come there is no fibre-to-fibre polyester recycling by re-melt-spinning? Is it due to degradation by i) hydrolysis...

24 June 2018 9,771 14 View

Whole mouse spinal cord immunolabeling in embryos/neonates. Easy?

Dear fellow scholars, We have a mouse line with eGFP and tdTomato labeled axons going from the brainstem to the spinal cord. We are going to clear the spinal cord (using iDisco) in embryos and...

21 March 2018 367 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View