Which is much better (especially for plant genome editing purpose), CRISPR/Cas9 or CRISPR/Cpf1?
I have seen research findings that indicate CRISPR/Cpf1 is more efficient ii genome editing when compared with CRISPR/Cas9. Please try to see the attached research finding.
Is it possible if undergraduate student doing a research with CRISPR/Cpf1?
Cpf1 is smaller and its corresponding gRNAs simpler. So it might be easier/faster to implement.
CRISPR/Cpf1 is smaller, requires one RNA molecule to cut DNA and can cut DNA at multiple sites, makes restriction overhangs which makes directional cloning much more easier. In my opinion, it is better than Cas9.
And yes, its ease of handling makes it suitable for any undergraduate researcher.
@ Ivan,
For your question: "Is it possible if undergraduate student doing a research with CRISPR/Cpf1?" Yes, of course. You can participate in the project. However, since this kind of experiments can take up to a few years, so most likely the advisor won't assign a whole project to a undergraduate student.
See this report: "Undergraduate students get rare chance to work with groundbreaking genetic engineering technique CRISPR"
https://www.utoronto.ca/news/undergraduate-students-get-rare-chance-work-groundbreaking-genetic-engineering-technique-crispr
Although CRISPR/Cpf1 has reported for a few years, but according to my observation, currently, most people are still using CRISPR-Cas9 for genome editing. In plant research, only a few papers published with CRISPR/Cpf1, comapred to the majority of papers using CRISPR/Cas9.
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