Could you tell me details about sphingolipid extraction from cell? What factors should be optimized for extraction?

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What are the fundamental ideas of Monte Carlo dimensionality estimation, compared to Rasch residuals PCA?

I read an interesting article from Christensen (2007): A Monte Carlo approach to unidimensionality testing in polytomous rasch models. But do you have some further thoughts or maybe other...

08 August 2024 4,245 0 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Can Langmuir constant related to bonding energy (Kl) and Freundlich exponent (1/n) indicative of ads intensity show opposite trends with temperature?

The adsorbate is Arsenic and the adsorbent is a soil

18 July 2024 332 8 View

Reversed flow at outlet due to the release of DFBI?

Hi everyone, I am working on a simulation involving restricted canal with ship using DFBI. I am facing reversed flow in my outlet boundaries as the DFBI is released (In 1.25s). Is there any...

17 July 2024 7,032 1 View

Cloning of flaviviruses is difficult i have noticed that cells loose their transformation ability after cloning fragments inside vector any advice?

As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...

11 July 2024 4,284 0 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Fast Question: Do hydrochloride salts run on a TLC plate?

Basically, I have deprotected a BOC, TPDMS-Protected sphingolipids using Acetyl Chloride, which gave a salt of deprotected sphingolipids. Can salts run on TLC?!

25 April 2024 2,971 1 View

It is possible to have fragmentations of sphingolipids in ESI mode?

Cerebroside and ceramide. Esi-ms in positive mode.

01 October 2023 2,031 1 View

Do you have PCR primers for detection of sphingolipids-producing bacteria in fecal sample?

I am going to detect abundance of sphingolipids - producing bacteria in fecal sample using qPCR. Do you have PCR primers for me or published PCR primer sequences? Many thanks!

22 October 2018 2,217 3 View

Do you have PCR primers for detection of sphingolipids-producing bacteria in fecal sample?

I am going to detect abundance of sphingolipids - producing bacteria in fecal sample using qPCR. Do you have PCR primers for me or published PCR primer sequences? Many thanks!

09 October 2018 7,495 3 View

What could explain poor extraction of sphingolipids from red blood cells?

I am trying to extract the sphingolipid d18:1 sphingosine from mammalian red blood cells and quantify it by HPLC-Fluorescence. I have tried to use the lipid extraction method in this paper...

05 June 2018 1,179 1 View

Intracellular C1P administration in vitro?

I want to treat my cells with ceramide 1-phosphate. I have reviewed the literature for possible ways but none seems clear enough. Does anyone have any recommendation on how to deliver c1p...

04 May 2018 3,950 0 View

How can sphingomyelinase produce plasma membrane products?

Some (e.g. Gulbins and Kolesnic, Oncogene 22:7070 (2003)) argue that sphingomyelin can be cleaved to ceramides in the plasma membrane, forming lipid rafts. This is outlined as a control pathway,...

14 March 2018 6,364 1 View

Would you help to extract Globotriaosylceramide (GB3) from cell?

I am working on cellular sphingolipids. I am looking for extraction of cellular Globotriaosylceramide (GB3) from cell?

06 July 2017 5,229 2 View

What type of solvent are better to analyze sphingolipids i.e ceramide, gb3, lacCer etc by HPLC ??

Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin

16 October 2016 872 1 View

What type of solvent are better to analyze sphingolipids i.e ceramide, gb3, lacCer etc by HPLC ??

Hello everyone, I have to develop a method to analyze sphingolipids by HPLC. I'm little confused about which solvents can I use for better results.Can anyone please suggest. Thanks, Sachin

09 October 2016 2,515 3 View