I am looking for customized tetramer synthesis for HLA DQ epitopes (MHC class-II), so could you please suggest sources please.
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Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...
20 June 2024 2,155 1 View
Hello everyone. I performed immunofluorescence staining to characterize MSCs that I isolated from the placenta. As expected, the results were CD90+, MHC-, and SOX2+. However, CD45, which should be...
28 February 2024 4,314 3 View
I already published an experimental research paper entitled "Neuroanthropology: Olfactory Recognition of the Self / Non-self by the Ancestral MHC: An EEG Study" in 2012 (doi:10.5539/ijb.v4n4p1)....
07 January 2024 2,508 4 View
My lab co-worker need a lot of T cell for his animal experiment, however one of the donor can't provide enough for downstream analyses, we thought to combine purified T cells from two donors in...
06 November 2023 7,188 0 View
Why is tetramer only used to test the interaction between T cell and peptide-MHC complex and not to test binding affinity between peptide and MHC?
12 May 2023 6,068 2 View
I used an antibody against H2-Kb SIINFEKL tagged with APC on Dendritic cells treated with nanocarriers carrying SIINFEKL peptide. After 3 hour incubation, the cells were washed 3 times with PBS...
10 February 2023 6,785 1 View
Hi. I have a question of the MHCII expression in mammal. What I know so far is that the expression of MHCII is codominant, alleles from both mom and dad will be expressed. However, I would like to...
25 July 2022 2,804 2 View
By using IEDB server
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Hello! I have a anti-mouse MHC Class I antibody (anti-H2Kb-H-2Db) that we are trying to prove works in blocking MHC I mediated interactions. We are using SIINFEKL and OTI mice as a model to do...
28 March 2022 2,340 0 View
I have taken B cell epitopes from consensus protein and overlapping T cell epitopes from B Cell epitopes having MHC class I and II binders. Is is enough if I just dock B cell epitopes with protein...
22 March 2022 4,499 5 View