How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

Any anti-HLA-B38/B39-specific antibodies?

Dear Fellow Researcher, I am working on HLA-B*38 and HLA-B*39 molecules and looking for the antibodies that can recognize these two MHC class I alleles. I do have W6/32 and HC10 antibodies that...

11 December 2019 4,712 1 View

Any anti-HLA-B38/B39-specific antibodies?

Dear Fellow Researcher, I am working on HLA-B*38 and HLA-B*39 molecules and looking for the antibodies that can recognize these two MHC class I alleles. I do have W6/32 and HC10 antibodies that...

02 December 2019 6,693 1 View

HLA A2 alpha chain not refolding properly, only B2 subunit is refolding. What is going wrong?

Hello, We are working on refolding HLA A2 in the presence of a known HLA A2-binding peptide. Our alpha chain is about 80% pure and our B2m is >90% pure. After rapid dilution in the refolding...

09 October 2019 6,649 5 View

In a diseased state, can a drug glide the immune-system from one MHC phenotype to another?

At different time points, if it is possible to observe shift in MHC-phenotype (MHC1 to MHC2 and/or vice-versa) due to pharmaceutical intervention during cancer treatment?

09 October 2019 7,229 3 View

Which primary antibody can recognize HLA-A24 in western blot?

Two most standard antibodies for MHC class I molecules detection by western blot HC10 and HCA2 do not recognize HLA-A24. During online search I came across two antibodies LY5.1 and D-2 that...

25 December 2018 8,337 3 View

Which primary antibody can recognize HLA-A24 in western blot?

Two most standard antibodies for MHC class I molecules detection by western blot HC10 and HCA2 do not recognize HLA-A24. During online search I came across two antibodies LY5.1 and D-2 that...

11 December 2018 8,247 4 View

How can I distinguish between mouse dendritic cells and monocytes?

I would like to identify mouse DC and distinguish them from monocytes in whole blood and tumor tissue mouse samples, by flow cytometry. May I do this using a panel with CD11c, CD11b, LY6C, F4/80,...

21 November 2018 2,113 3 View

How can I distinguish between mouse dendritic cells and monocytes?

I would like to identify mouse DC and distinguish them from monocytes in whole blood and tumor tissue mouse samples, by flow cytometry. May I do this using a panel with CD11c, CD11b, LY6C, F4/80,...

10 November 2018 9,091 4 View