I want to clone about 300-600 bp DNA fragment from yeast genome. Since there have many high-fidelity DNA polymerase i.e. Phusion, KOD HiFi, Q5 etc.
Colud you suggest me which is the most proper DNA polymerase that provide high-fidelity, high yield , cost effective, for cloning 300-600 bp DNA fragments?
HI Tatpong,
I have had fantastic success with Platinum Taq HiFi for both short and long fragments.
Hello
I suggest you use phusion high fidelity Taq from thermo scientific.
Hi, Phusion polymerase from NEB never fails me to have result even with bigger fragments like 1kb-2kb.
As many others wrote I would pick the Phusion polymerase, it always work in our hands.
Dear Preuett, Liger, De, Khoury, Cantos, and Moeller,
Thank you for your kind suggestion.
Suggest trying NEB's Vent polymerase - high proofreading; it has been available for many years now, perhaps because it is so reliable.
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