If I coat protein for indirect ELISA overnight , Should I add PMSF into coating buffer or not?

I express recombinant protein in yeast. After that harvest cell and resuspend cell in Phosphate Buffer Saline (PBS) buffer with 1 mM PMSF (as protease inhibitor), then break it by glass bead and...

08 September 2016 6,964 3 View

Why In Vitro (Cell-free) recombinant protein synthesisis not used instead of microorganisms in the industrial scale?

11 December 2015 7,865 9 View

Could anyone suggest the Deletion of which gene can reduce hyperglycosylation in Pichia pastoris?

In yeast, we face to the problem of recombinant protein expression because of its ability in hyperglycosylation. If I aim to reduce the hyperglycosylation without affect to the growth of the Yeast...

05 June 2015 7,421 4 View

What is the meaning of Em on E.coli-Yeast shuttle vector?

I read a paper Highly-efficient electrotransformation of the yeast Hansenula polymorpha (Faber, 1994) then I doubt that what is the meaning of Em or something on the plasmid? For me I think maybe...

04 May 2015 8,904 2 View

Can S. cerevisiae URA3 gene can function in H. polymorpha NCYC495?

I constuct plasmid that contains FMD promoter, MOX terminator, HARS1, and URA3 gene from S. cerevisiae, then transform by LiAc method to H. polymorpha NCYC495 for three times. But every time don't...

04 May 2015 166 3 View

Could anyone suggest why I get very little amount of plasmid DNA after extraction from E. coli.?

I already obtained two plasmid that harboring gene synthesis A or B. First is pUC57 harboring gene A (size of insert 1.5 kb). Second is pUC57 harboring gene B (size of insert 1.6 kb). Then, I...

03 April 2015 7,851 14 View

Does anyone ever use PDM (Plasmid DNA Medium) for E.coli plasmid preparation?

I read from Bitesize Bio website that Michael K. Danquaha, and Gareth M. Fordea (2007) published a paper for plasmid DNA production. This PDM can give a higher yield than the other E. coli medium,...

02 March 2015 4,849 3 View

What is the chemical for washing and removing an undesired or contaminated Plasmid DNA?

Now, I'm in the step of plasmid construction in E. coli. But I found a big problem because of an undesired or contaminated plasmid DNA (some one called Ghost plasmid) in my transformants (more...

01 February 2015 9,007 6 View

Does anyone suggest pBluscript II SK(+) or related with Kanamycin resistance marker

Does anyone suggest a plasmid that be a "pBluescript II SK(+) with Kanamycin resistance marker (instead of AmpR)" or relates. Best regards, Tatpong

11 December 2014 1,574 3 View

Could you suggest high fidelty DNA polymerase for cloning about 300-600 bp DNA fragments?

I want to clone about 300-600 bp DNA fragment from yeast genome. Since there have many high-fidelity DNA polymerase i.e. Phusion, KOD HiFi, Q5 etc. Colud you suggest me which is the most proper...

09 October 2014 3,546 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View