Christian Cantos

4 Questions 32 Answers 0 Followers

Questions related from Christian Cantos

What is the best way for the colony PCR to work efficiently?

I am having a problem in doing colony PCR. Sometimes it works, sometimes it won't. Basically, what I do is to touch the edge of the colony with a toothpick and then submerged it to the PCR...

10 October 2014 3,825 25 View

Are there any methods for efficient isolation of plasmid DNA in Agro that are good for sequencing and transformation?

I am using an Invitrogen Midi prep Kit, however, I always get a very low concentration even though my initial volume is around 10-25ml.

07 July 2014 2,846 3 View

Where can I see a site for putative genes in rice?

I am designing a guide RNA for Crispr to target specific genes in rice that when silenced can have a good effect on the stability of rice, such as disease-resistance.

06 June 2014 6,663 3 View

How to isolate a good number of rice protoplast cells to be used in PEG transformation?

I wanted to try the activity of our TALEN constructs in rice protoplast first before we used rice immature embryo for transformation, and I am finding difficulty in isolating good number of...

06 June 2014 8,175 2 View