How do I interpret p and q in the corARMA correlation structure?

I understand that p is the autoregessive order and q is the moving average, but I don't know how to interpret what does it means when it is (2,3) or (2,2) for example. Can anyone give me a simple...

09 October 2018 6,260 0 View

Do you have any recomendations for a MALDI-TOF MS instrument?

I am exploring the possibility of buying of a Maldi-Tof MS instrument for my lab. I am familiar only with Bruker Maldi-ToF MS. My main application is characterization of novel peptides so accuracy...

02 March 2018 1,740 6 View

More sequencing is needed to profile functional diversity than phylogenetic diversity, is this true?

There is a commentary in Langille et al., 2013: Predictive functional profiling of microbial communities using 16S r RNA marker gene sequences. "More sequencing is needed to profile functional...

27 March 2017 6,996 3 View

Are tandem repeats of rDNA target for SSR markers?

15 February 2017 2,539 1 View

Is C value paradox applied only for genome size, or to genome complexity, as well?

C value paradox describes the lack of relationship between genome size and organismal complexity (Buchanan Biochemistry and Molecular Biology of Plants, 2nd Edition p. 405). I wonder if this...

08 February 2017 9,825 4 View

Can i infect BHK-21 with poliovirus strain?

06 October 2016 5,643 3 View

How to differentiate if it is a new type of protein or a new variant of the same type?

I am wondering what is the % or number of Aa different to consider a protein as a new type or new variant?. I am working with new proteins (Kazal-type protease inhibitors). If you can suggest a...

03 March 2016 7,361 3 View

How to differentiate if it is a new type of protein or a new variant of the same type?

I am wondering what is the % or number of Aa different to consider a protein as a new type or new variant?. I am working with new proteins (Kazal-type protease inhibitors). If you can suggest a...

02 March 2016 3,904 4 View

Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

25 September 2014 3,151 3 View

Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

08 September 2014 1,408 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View