I am doing a renal fibrosis study using folic acid model of C57BL/6J mice. Our initial dose was 250mg/kg bwt. which we reduced to half (125mg/kg bwt) once we saw more animal death after injection. Again, after injection of reduced dose, animal death did not reduce significantly instead death occurred on 3rd day of FA injection while with higher dose animal death was observed on 2 day after FA injection. Any expert opinion would be highly appreciated.

Thanks! in advance