Could you please advise the current efficient protein validation method?

More Kathiresan Purushothaman's questions See All

Could you please advise me that any suitable techniques for this experiment which will be done shortly?

I am planning to perform the functional study of gene whuch is supplied maternally, so morpholino-mediated knockdown assay won't work. Also about the CRISPR mediated knockout study need to wait...

27 December 2020 731 1 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to generate adsorption isotherms ?

We are doing a project on adsorption of hexavalent chromium using agricultural wastes. I would want to generate adsorption isotherms for our data. Would I be able to know the procedure for the...

24 May 2019 1,655 13 View

Which is the good and required structure that need to be designed to solve current problems in a society?

I am a final year UG student, this semester we need to do design project. But we have learnt only member design in our curriculum, so I am looking for a structure by designing that we can get a...

15 June 2018 1,986 7 View

How can I address the issue of a significant change in the Control group?

A study on effect of a particular yogic maneuver was carried out in our department. It involved two groups, control and intervention group( n = 50 in each). It so happened that, at the end of...

26 April 2015 4,409 3 View

What are the alternate materials for machine bases other than cast Iron ?

Material science are developing to great extent kindly share the information on alternative material for cast iron. I came to know that some of the machine bases are made of polymer concrete,...

04 February 2015 456 13 View

Which is the best element choice for muliti layered FRP material in ANSYS ?

I have to conduct FE analysis of aircraft structure of GFRP material which is of multi layered kindly suggest me how to do modelling of multi layered elements with different material properties in...

18 December 2014 9,482 4 View

What is the equivalent concentration of lactose to induce T7 based recombinant expression instead of IPTG?

Hi Guys, Lactose is a metabolisable sugar molecule and affected by inducer exclusion and catabolite repression. Any experience of using lactose as inducer instead of IPTG to improve recombinant...

10 November 2014 4,662 3 View

How will co efficient of friction change with respect to the change in contact area ?

Does the co efficient of friction depend only on materials in contact? In frictional clamps the co coefficient of friction plays a major role, as co efficient of friction directly depends on the...

09 October 2014 1,469 19 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Kathiresan Purushothaman

Thank you very much for your advice

Roger Davis

Hi,

I agree with Wael R Abdel-Fattah , mass spectrometry technologies can meet your research needs.

Stephanie Misquitta

Mass spectroscopic analysis is a suitable technique, and a starting point to plan further validation.

Thank you for your suggestions

Qin Fu

Hi Kathiresan, you can try PRM/MRM based on UPLC-MS/MS for validation of your proteins if antibodies are not available