Could you help in prevention of sodium phytate from precipitation in the enzymatic reaction mixture at pH 5.5 using sodium acetate buffer?

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Is it possible to get two isoenzymes with same molecular weight?

Is it possible to get two isoenzymes with same molecular weight? As, after doing anion exchange chromatography on a DEAE-cellulose column, two peaks of the enzyme activity were resolved by the...

07 August 2019 2,745 4 View

Is it possible to acknowledge the language editing person in the acknowledgement part in the manuscript?

06 July 2016 1,603 3 View

How I can calculate the micro moles produced from the enzymatic reaction?

If I want to say one unit of enzyme is the amount of enzyme that able to produce one micro mole of the product, How I can calculate the micro moles produced from the enzymatic reaction? Thanks

10 November 2015 2,803 5 View

Please can any one help me to get the molcular formula and chemical structure of Acid Blue C.I. 221, CAS No 12219-32-8

I want to know the chemical structure and molecular formula of Acid Blue dye CI 221

08 September 2014 987 0 View

What are the best rules for regeneration of strong and weak ion exchange columns?

How do I know that my column is regenerated effectively after usage?

31 December 2012 4,546 4 View

What is the difference between micromoles and micromolar; and how I can calculate the Kcat for purified enzymes?

Km, Vmax

09 October 2012 8,116 8 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View