Could you help in prevention of sodium phytate from precipitation in the enzymatic reaction mixture at pH 5.5 using sodium acetate buffer?

More Abdelmageed Othman's questions See All

Is it possible to get two isoenzymes with same molecular weight?

Is it possible to get two isoenzymes with same molecular weight? As, after doing anion exchange chromatography on a DEAE-cellulose column, two peaks of the enzyme activity were resolved by the...

07 August 2019 2,852 4 View

Is it possible to acknowledge the language editing person in the acknowledgement part in the manuscript?

06 July 2016 1,734 3 View

How I can calculate the micro moles produced from the enzymatic reaction?

If I want to say one unit of enzyme is the amount of enzyme that able to produce one micro mole of the product, How I can calculate the micro moles produced from the enzymatic reaction? Thanks

10 November 2015 2,901 5 View

Please can any one help me to get the molcular formula and chemical structure of Acid Blue C.I. 221, CAS No 12219-32-8

I want to know the chemical structure and molecular formula of Acid Blue dye CI 221

08 September 2014 1,073 0 View

What are the best rules for regeneration of strong and weak ion exchange columns?

How do I know that my column is regenerated effectively after usage?

31 December 2012 4,678 4 View

What is the difference between micromoles and micromolar; and how I can calculate the Kcat for purified enzymes?

Km, Vmax

09 October 2012 8,341 8 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View