Could you help in prevention of sodium phytate from precipitation in the enzymatic reaction mixture at pH 5.5 using sodium acetate buffer?

Could you help in prevention of sodium phytate from precipitation in the enzymatic reaction mixture for phytase assay at pH 5.5 using sodium acetate buffer? Also is it possible to use citrate...

05 June 2018 557 2 View

Is it possible to acknowledge the language editing person in the acknowledgement part in the manuscript?

Is it possible to acknowledge the language editing person in the acknowledgement part in the manuscript?

06 July 2016 1,603 3 View

How I can calculate the micro moles produced from the enzymatic reaction?

If I want to say one unit of enzyme is the amount of enzyme that able to produce one micro mole of the product, How I can calculate the micro moles produced from the enzymatic reaction? Thanks

10 November 2015 2,803 5 View

Please can any one help me to get the molcular formula and chemical structure of Acid Blue C.I. 221, CAS No 12219-32-8

I want to know the chemical structure and molecular formula of Acid Blue dye CI 221

08 September 2014 987 0 View

What are the best rules for regeneration of strong and weak ion exchange columns?

How do I know that my column is regenerated effectively after usage?

31 December 2012 4,546 4 View

What is the difference between micromoles and micromolar; and how I can calculate the Kcat for purified enzymes?

Km, Vmax

09 October 2012 8,116 8 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View